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KMID : 0380019940090020098
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 2 p.98 ~ p.102
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A Study on the Chromatographic Separation of Proteins Using Fibrous Beds(Ⅰ)
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Daynes, Raymond A.
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Abstract
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A bed configuration wherein sheets of modified fibrous polyethylene are potted within a Millipore Filter Cartridge matrix has been developed. Polyethylene fibers form sturdy beds, but the native hydrophobicity and inertness of polyethylene have precluded their use in protein chromatography. The polyethylene fibers used in this system were modified by plasma oxidation and further derivatization. The resulting fibers are hydrophilic, bind protein reversibly and serve as an anion-exchange stationary phase. Separation of Bovine Serum Albumin on this bed, as well as results of basic studies on capacity and reversibility of binding within a fibrous bed and experimental data handling system are shown.
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